ABSTRACT
OBJECTIVE: To observe the effects of recipes for replenishing qi and activating blood on p16, p21, proliferating cell nuclear antigen (PCNA), cyclin D1 and cyclin E gene expressions in the liver of aging rats. METHODS: A recipe for replenishing qi and a recipe for activating blood were administered to aging rats respectively, and the effects of the above recipes on the expressions of senescence related genes (p16, p21, PCNA, cyclin D1 and cyclin E) were examined by RT-PCR and Western blotting methods. RESULTS: The expressions of p16, p21 and cyclin D1 mRNAs and proteins in the liver of the untreated aging rats were up-regulated, while the expressions of PCNA and cyclin E mRNAs and proteins decreased. As compared with the untreated aging rats, both recipes could down-regulate the expressions of cyclin D1 mRNA and protein and up-regulate the expressions of cyclin E mRNA and protein, but had no obvious effects on the expressions of mRNAs and proteins of p16, p21 and PCNA. CONCLUSION: Recipes for replenishing qi and activating blood can improve the liver cell proliferation of aging rats via down-regulating the expressions of cyclin D1 mRNA and protein and up-regulating the expressions of cyclin E mRNA and protein.
ABSTRACT
Object To study the effect and mechanism of heart beneficial recipe (HBR) on ? amyloid induced neuronal apoptosis Methods In order to establish neurotoxic model, the primary cultured rat hippocampal neurons were treated with 25 ?mol/L aggregated ? amyloid fragment 1 42 (A? 1 42) Neuronal survival was assessed by viable counting after double stained with fluorescein diacetate (FDA) and propidium iodide (PI) Apoptosis of neurons was determined by Hoechst 33258 staining and terminal transferase deoxyuridine nick end labeling (TUNEL) staining The protein expression of Caspase 3 was examined by immunocytochemical SABC method The transcription of mRNAs related to bcl 2, bax, c jun and c fos gene were observed by Northern Blot or RT PCR method Results In the A? 1 42 treated neurons, the number of viable cell was decreased Hoechst 33258 staining showed condensation of nuclear chromatin and nuclear fragmentation TUNEL staining revealed that the number of positive neuron was increased The expression of Caspase 3 protein was elevated The expression of bcl 2 mRNA was decreased, but bax and c jun mRNA were inereased After HBR treatment, the above indexes were improved Conclusion HBR could regulate the expression of apoptosis associated gene and Caspase 3, attenuate the neurotoxic action of A? 1 42 and improve neuronal viability via suppressing apoptotic process